AFL injury list:
| Player | Injury | Update |
| Jack Carroll | Finger | "He fractured his thumb in training, he's probably going to miss 3-4 weeks." Update: 6 April |
| David Cuningham | Concussion | Test for selection. Update: 13 April |
| Charlie Curnow | Knee | On track for return in second half of 2021. Update: 11 March |
| Tom De Koning | Back | "It looks like we'll see him back out there after Round 6." Update: 30 March |
| Zac Fisher | Ankle | "He's got some bone stress in his ankle. We think he's going to miss around six weeks." Update: 30 March |
| Brodie Kemp | Foot | "We feel he needs that 5-6 week block of football conditioning to be available." Update: 13 April |
| Caleb Marchbank | Knee | Out for remainder of season. |
| Jack Martin | Knee | "Classic PCL mechanism... that's going to require him to be on the sidelines for six weeks." Update: 30 March |
| Oscar McDonald | Back | "He's got a bone stress injury in his lower back... he could be up to three months out." Update: 13 April |
| Nic Newman | Knee | "We feel he needs another 2-3 weeks of skills conditioning to be ready to play." Update: 13 April |
| Jack Silvagni | Shoulder | Unavailable for Round 5. Recovery plan to be determined later in week. Update: 13 April |
| Zac Williams | Calf | Test for selection. Update: 13 April |
AFLW injury list:
| Player | Injury |
| Mimi Hill | Knee |