Scans have confirmed Carlton defender Ciaran Byrne ruptured the anterior cruciate ligament in his right knee during Saturday night's match against Collingwood at the MCG.
The injury will sideline the 21-year-old for the rest of the season.
Head of Football Andrew McKay said confirmation of the long-term injury was unfortunate for the developing talent.
"Long term injuries are always disappointing, particularly as Ciaran was having a really solid season so far," McKay said.
"He will undergo surgery in the coming weeks and will commence his rehabilitation process not long after that.
"Ciaran is a resilient young man and will have the club's full support throughout this process."